Table 3 Primer sequences and reaction conditions for the BGS, MSP and RT-PCR experiments
Primer | sequence | Size (bp) | Temperature (°C) |
|---|---|---|---|
STK31-BGS | F: 5ʹ-TTTTTAAAGTTATAGTTTGAAGTTTTG- 3ʹ | 499 | 50 |
R: 5ʹ- ACATCTAACACCCCTCTAAAATAAC - 3ʹ | |||
STK31-M | F: 5ʹ- TTGTTACGTGATTTTCGTTAATATC - 3ʹ | 149 | 51 |
R: 5ʹ-TAAACCCACATACTAAACTTTCGAC - 3ʹ | |||
STK31-U | F: 5ʹ-GTTATGTGATTTTTGTTAATATT - 3ʹ | 147 | 51 |
R: 5ʹ-TAAACCCACATACTAAACTTTCAAC - 3ʹ | |||
STK31-RT | F: 5′-AGAGGAATATGAGATGCTAACTA - 3′ | 111 | 60 |
R: 5′- GTAAGGAGACCACCAGAG - 3′ | |||
GAPDH | F: 5′- GATGACCTTGCCCACAGCCT - 3′ | 303 | 60 |
R: 5′- ATCTCTGCCCCCTCTGCTGA - 3′ |