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Fig. 4 | Virology Journal

Fig. 4

From: S1 domain of the porcine epidemic diarrhea virus spike protein as a vaccine antigen

Fig. 4

Deglycosylation analysis of the purified recombinant S1 proteins. S1 proteins purified from cell culture media of HEK 293 T cells or baculovirus infected Sf9 cells were treated with deglycosylation enzymes PNGase F or O-Glycosidase or left un-treated. Proteins were resolved on 10 % SDS-PAGE and analysed by Western blotting using anti-his antibody. Numbers on the left indicate the protein molecular marker size in kDa

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