Table 3 Primers used for complete genome sequence amplification of strain GD
Primera | Location (bp) | Upstream primer | Downstream primer | Length (bp) |
|---|---|---|---|---|
1 | 22–1571 | TATATATCTATTGCACTAGCC | AGTCAGACAGACAACACGCT | 1549 |
2 | 1025–1677 | GCAGACTTGTTGGTGAGGTTA | CACAAGTTCCGAAACACTAAA | 652 |
3 | 1530–2687 | TGGAGGGACATCTTTGCTAT | TCTGTCTCAACTTCAATGGG | 1157 |
4 | 2566–3961 | TGGTGAAACTACTGTGAAGG | CACACCATCTACAAGAACAT | 1395 |
5 | 3642–5168 | TGTTAACGCCGCAAATGAG | GGCAACTTGGAATCTTCCT | 1527 |
6 | 4873–6504 | TAAAAAGAGTAAGAGCAAGA | AGGAGACATAAGTGTATTTTG | 1631 |
7 | 6534–7920 | CGTCTACACTAACTCAGGCTA | CCTGACTCCACTAGGTTGAA | 1386 |
8 | 7890–9260 | GAAATTGTTGGTTACACCCAC | TAGAACGCATAGTAACGGGG | 1370 |
9 | 9247–10664 | TCAGTAGGCGTTTGAAAGG | ATAGGCAACACACGGTCA | 1417 |
10 | 10594–12110 | GGCATAGGTTGGATGTTTACT | CACAGAAGCCCCTCCGTAA | 1516 |
11 | 11954–12523 | GTCTTACAGTCTAAAGGAT | AGCACAGTTACGCTTCAAAT | 569 |
12 | 12418–13418 | ACTTAGACAACCAAAACCCTT | TCATAATAACACCGAGTTCCT | 1000 |
13 | 13261–13978 | CCCTCCTCAAGTATGATTAT | ATAGTGGGCAGGACATTCTT | 717 |
14 | 13865–15044 | TATTTTGTTTAGAGGTGACG | AGGAATAGTCAATAAGCAT | 1179 |
15 | 14712–16138 | CTGATTCTAAGTGTTGGGTT | TCCTTTGAGGTACTATGCGA | 1427 |
16 | 15516–16465 | ACTCGCTAAGACGCTTTGCT | TTAGGACAACGGTAACACTTC | 950 |
17 | 16283–17509 | GTGACATTCTATTGGTTGAT | ACAGACTTGTTCCTTGCCT | 1226 |
18 | 17503–19024 | TCACTTGAGAGCTTTGT | TAAACATACAGATTCGCT | 1521 |
19 | 18628–19634 | ATCAAACAACTCTGCCTACA | CCACATTCATCATAATACCACT | 1007 |
20 | 18945–20442 | AAGCGGTATYCNTATGTAGA | ATAGTRCAVACAAAAKRGTCA | 1498 |
21 | 20189–20578 | TTATTTGGGTGACAGTGG | ACCACTAATAATACCAACAG | 389 |
22 | 20437–22232 | AAGGTTAATCCCTGTGAAG | AGTYTCVGTAAGAATAGCA | 1795 |
23 | 21873–22919 | AAGGTTAATCCCTGTGAAG | AGTYTCVGTAAGAATAGCA | 1047 |
24 | 22723–23837 | CTTTTGCHACTCAGATDCA | AGATTTCTTACCACACTTACT | 1115 |
25 | 23791–24992 | AGGGGCTTAATGACTCTCTT | CTGACCTTCACAATAAAGAAC | 1201 |
26 | 24796–25894 | GCAGCGATAATACTTACAGT | TCTGCTTGTCCTGCTTTGT | 1099 |
27 | 25121–26453 | GTGACCGAAGCGGAAATAA | TCAGAGGAATGAAGTCCCAAC | 1333 |
28 | 26891–27706 | GGTGATTCTCAAGATGGTAT | GCTCTAACTCTATACTAGCCT | 1416 |