Fig. 1

Construction and identification of rL-VSV-G. a Schematic representation of the rLaSota genome and VSV G inserted between the P and M genes. b Western blot assay of expression of VSV G. BHK-21 cells were infected with rLaSota or rL-VSV-G at a MOI = 1. After 24 h, cells were collected and lysed, the cell lysate was separated by SDS-PAGE and immunoblotted with mouse anti NDV antibodies or VSV G monoclonal antibody. c Immunofluorescence analysis of VSV G protein expression. BHK-21 cells were infected with rLaSota or rL-VSV-G at a MOI = 0.01. After 24 h, the cells were fixed and then stained with chicken anti-NDV antibody or VSV G monoclonal antibody followed by incubation with FITC-conjugated goat anti-mouse antibody or a TRITC-conjugated rabbit anti-chicken antibody