Fig. 1From: The M, F and HN genes of genotype VIId Newcastle disease virus are associated with the severe pathological changes in the spleen of chickensSchematic representation of the clone strategy for the full-length antigenomic cDNA clone of Herts/33. Ten overlapped cDNA fragments spanning the entire genome were generated and cloned into the transcription vector TVT (0.0). Six unique restriction sites (italicized) and three additional sites (boxed) were used to sequentially assemble these fragmentsBack to article page