Fig. 2
From: Identification of four novel group-specific bluetongue virus NS3 protein B-cell epitopes
Characterization of NS3-reactive mAbs. a. Each mAb was tested by WB for reactivity against recombinant MBP-NS3 protein, native BTV15 NS3 protein in BTV15-infected BHK-21 cells, and its corresponding identified peptide epitope. M: PageRulerTMPrestained Protein Ladder; lane 1, lysate of uninfected BHK-21 cells; lane 2, lysate of BTV15-infected BHK-21; lane 3, MBP-only protein; lane 4, recombinant MBP-NS3 protein; lane 5 (and lane 6): identified linear peptide epitopes for each mAb. mAbs used are labeled at the lower right hand corner of each panel. mAb 1B5, 2B12 and 4H8 were evaluated against MBP-NS3-26; 2G9 was evaluated against MBP-NS3-11; 3D8 was evaluated against MBP-NS3-4 and MBP-NS3-5. b. BHK-21 cells were infected with BTV15 and used to evaluate mAb reactivity by IFA. Uninfected BHK-21 cells served as negative control for each mAb. mAbs used are labeled at the lower left hand corner of each panel