Fig. 1
From: Identification of four novel group-specific bluetongue virus NS3 protein B-cell epitopes
Expression and purification of recombinant BTV15-NS3 protein. a: SDS-PAGE analysis of recombinant MBP-NS3 protein produced in E. coli. M, PageRulerTMPrestained Protein Ladder (Fermentas, Canada); Lane 1, expression of MBP-only protein; lane 2, MBP-NS3 protein before induction; lane 3, supernatant of cell lysate of recombinant MBP-NS3 protein after ultrasonication; lane 4, cell lysate pellet of recombinant MBP-NS3 protein after ultrasonication; lane 5: purified recombinant MBP-NS3. b: SDS-PAGE analysis of recombinant HIS-NS3 protein in E. coli. M: PageRulerTMPrestained Protein Ladder (Fermentas, Canada); lane 1, expression of HIS-only protein; lane 2, HIS-NS3 protein before induction; lane 3, supernatant of cell lysate of recombinant HIS-NS3 protein after ultrasonication; lane 4, cell lysate pellet of recombinant HIS-NS3 protein after ultrasonication; lane 5, purified recombinant HIS-NS3. c: WB analysis of recombinant MBP-NS3 protein and HIS-NS3 protein. M, PageRulerTMPrestained Protein Ladder (Fermentas, Canada); Lane1, recombinant MBP-NS3 before purification; lane 2, purified recombinant MBP-NS3; lane 3, recombinant HIS-NS3 before purification; lane 4, purified recombinant HIS-NS3. Membranes were probed with either an HRP-conjugated anti-MBP (left panel) or anti-HIS antibody (right panel)