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Figure 1 | Virology Journal

Figure 1

From: RNA interference-based resistance in transgenic tomato plants against Tomato yellow leaf curl virus-Oman (TYLCV-OM) and its associated betasatellite

Figure 1

Resistance to infection by TYLCV-OM and TYLCV-OM/ToLCB-OM imparted by the transient expression of the hpRNAi construct. Tomato plants inoculated with TYLCV-OM and the hpRNAi construct (A and B), TYLCV-OM/ToLCB-OM and the hpRNAi construct (C and D), TYLCV-OM (E) or TYLCV-OM/ToLCB-OM (F). N. benthamiana plants inoculated with TYLCV-OM and the hpRNAi construct (G), TYLCV-OM/ToLCB-OM and the hpRNAi construct (H), TYLCV-OM/ToLCB-OM (I) or TYLCV-OM (J). Photographs of plants were taken at 30 dpi. Southern blot probed for the presence of TYLCV-OM sequences (K). The DNA samples run on the gel were extracted from a healthy, non-inoculated N. benthamiana plant (lane 2), N. benthamiana plants inoculated with TYLCV-OM (lane 3), hpRNAi and TYLCV-OM (lanes 7 and 8) and tomato plants inoculated with TYLCV-OM (lane 4) or hpRNAi and TYLCV-OM (lanes 5 and 6). The sample in lane 1 consisted of 50 ng of TYLCV-OM plasmid.With the exception of lane 1, approx. equal amounts (10 μg) of total DNA extract was loaded in each case. Southern blot probed for the presence of ToLCBV-OM sequences (L). The DNA samples run on the gel were extracted from a healthy, non-inoculated N. benthamiana plant (lane 1) and N. benthamiana plants inoculated with TYLCV-OM/ToLCB-OM (lane 2) or hpRNAi and TYLCV-OM/ToLCB-OM (lanes 6 and 7) and tomato plants inoculated with TYLCV-OM/ToLCB-OM (lane 3) or hpRNAi, TYLCV-OM/ToLCB-OM (lanes 4 and 5). Approx. equal amounts (10 μg) of total DNA extract were loaded in each case. The positions of the viral single-stranded (ss) and supercoiled (sc) replicative DNA forms are indicated.

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Virology Journal

ISSN: 1743-422X