Figure 2

GRA reduces rotavirus yields when added post--infection. MA104 cells were infected with rotavirus strain RRV at a multiplicity of infection of 3 pfu/cell. 25 μg/mL GRA was added to the cultures either six hours pre-infection and then removed for the remaining time (pre) or was present continuously in the media throughout the course of infection (pre/post). For post-infection treatments, GRA was added to the cultures following the one hour virus adsorption period and maintained in the media throughout the infection. Total virus was harvested from cells and supernatant, and yields of infectious virus were determined by IFA. Data shown are representative of two experiments with triplicate samples in each experiment. Analysis for significance was performed using a two--‒tailed Student’s t test. Errors indicate SEM. *p = 0.006.