Genes | Analysis | Types | Sequences | Annealing temperature for LMD and RT-PCR analysis | Accession number |
---|---|---|---|---|---|
meq | real-time PCR | Forward | 5′-GTCCCCCCTCGATCTTTCTC-3′ | 60°C | AY362736 |
real-time RT-PCR | Reverse | 5′-CGTCTGCTTCCTGCGTCTTC-3′ | |||
LMD and RT-PCR | |||||
β-actin | real-time PCR | Forward | 5′-GAGAAATTGTGCGTGACATCA-3′ | 55°C | L08165 |
Reverse | 5′-CCTGAACCTCTCATTGCCA-3′ | ||||
β-actin | real-time RT-PCR | Forward | 5′-CCAACTGGGATGATATGGAGAAG-3′ | 58°C | NM205518 |
LMD and RT-PCR | Reverse | 5′-AGGCATACAGGGACAGCACA-3′ | |||
PD-1 | real-time RT-PCR | Forward | 5′-GGACTACGGTGTGCTGGAGTT-3′ | 60°C | XM422723 |
LMD and nested RT-PCR (2nd PCR) | Reverse | 5′-TCTTTCCTCGCTCTGGTGTG-3′ | |||
PD-1 | LMD and nested RT-PCR (1st PCR) | Forward | 5′-ACACCCTGGCGGAGGTGAAG-3′ | 60°C | XM422723 |
(1st PCR) | Reverse | 5′-TGCCAGGTCCTCTGATCGTTGTG-3′ | |||
PD-L1 | real-time RT-PCR | Forward | 5′-TTCAGGGACGGATAAAGCTG-3′ | 60°C | XM424811 |
LMD and nested RT-PCR (2nd PCR) | Reverse | 5′-CGTCTCTGAGCTTCACGTTG-3′ | |||
PD-L1 | LMD and nested RT-PCR (1st PCR) | Forward | 5′-GGGACTTAAGCGTCATCTGGGAA-3′ | 58°C | XM424811 |
(1st PCR) | Reverse | 5′-GGTAAAGCCCCGCGTCTCTG-3′ | |||
IFN-γ | real-time RT-PCR | Forward | 5′-CTCCCGATGAACGACTTGAG-3′ | 58°C | NM205149 |
Reverse | 5′-CTGAGACTGGCTCCTTTTCC-3′ |