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Table 5 Results of JEV detection by ELISA assay and RT-PCR for 60 clinical samples

From: Development and application of an antigen capture ELISA assay for diagnosis of Japanese encephalitis virus in swine, human and mosquito

 

RT-PCRb

 
 

Positive

Negative

Total

ELISAa

   

   Positive

14

0

14

   Negative

6

40

46

Total

20

40

60

  1. Compared to RT-PCR, the positive coincidence rate of ELISA:
  2. 14/(14 + 6) × 100% = 70%; the negative coincidence rate of ELISA: 40/(40 + 0) × 100% = 100%; the total coincidence rate: (14 + 40)/60 = 90%;
  3. a Antigen capture ELISA assay
  4. b Reverse transcription polymerase chain reaction