Figure 2

The effect of HPV oncogenes on the mRNA levels of selected genes in HFK cells. Generation of cell lines and induction of differentiation was performed as described in the legend to Figure 1. Relative Quantification (RQ) values were obtained by real-time RT-PCR analysis to see the effects of HPV 16 oncogenes on the level of TERT (A), IVL (B), or TG1 (C) mRNA in HFK cells. The RQ value of proliferating cells transduced by LXSN was set to 1, and other values are shown relative to this. Each reaction was performed in triplicate at least three times. Representative graphs are shown, with error bars indicating standard deviation.