Table 1 Primers used for LAMP
Primer | Type | Length | Sequence |
|---|---|---|---|
F3 | Forward outer | 18-nt | 5’- TATCTGGTTCTGGCGGAC -3’ |
B3 | Backward outer | 23-nt | 5’- AGCATATTTCTCTTTCTAGTCTC -3’ |
FIP | Forward inner (F1c + F2) | 46-mer(F1c:25-nt, F2:21-nt) | 5’- TAGCAGATGCTTCCATATGGTAATT-GGGCAGATATTGTATACAGGA -3’ |
BIP | Backward inner(B1c + B2) | 43-mer (B1c:22-nt, B2:21-nt) | 5’- TTCTAAGTTGGCCTACTTGCCC-ATGAAGGATACACGTGAACAC -3’ |
LF | Loop Forward | 23-nt | 5’- ACAACTCCTTAACGATCACCGAA -3’ |
LB | Loop Backward | 25-nt | 5’-ATCAGGAAGGTGATAAATGATGGAC -3’ |