Figure 3From: Development and evaluation of a loop-mediated isothermal amplification assay for the rapid detection of porcine cytomegalovirus under field conditionsSensitivity of the LAMP and PCR. (A) Sensitivity of LAMP. (B) Sensitivity of PCR. M, DL-2000 DNA marker; 1–5, reaction carried out using 10-fold serial dilutions of standard PCMV DNA (1.0 × 103 copies/μL): 1: 1.0 × 10–1 copies/μL, 2: 1.0 × 100 copies/μL, 3: 1.0 × 101 copies/μL, 4: 1.0 × 102 copies/μL, 5: 1.0 × 103 copies/μL, respectively; Lane 6: –, negative control, Lane 7: +, positive control. All products were electrophoresed on 2% agarose gels and stained with ethidium bromide.Back to article page