Figure 5

HCVcc infection kinetics in hepatoma cells. Huh7, PLC, Hep3B and HepG2-CD81 cells were infected with JFH-1 HCVcc at an MOI of 0.01 FFU/cell. Culture supernatant and intracellular RNA were collected at the indicated times p.i. (A) Intracellular HCV RNA was analyzed by RT-qPCR, normalized to GAPDH and is displayed as HCV copies/μg total RNA (means ± SD for triplicate samples). (C). On day 5 p.i., parallel cultures of infected cells were fixed and stained for HCV E2 [39] (magnification, 100×). (C) Infectivity titers, expressed as FFU/ml, were determined on naïve Huh7-1 cells. The dashed line represents the lower limit of detection of the assay (10¹ FFU/ml).