Figure 6
From: Polar release of pathogenic Old World hantaviruses from renal tubular epithelial cells
Infection of MDCKII cells with PUUV. MDCKII cells were grown on coverslips and infected with PUUV. Infection was analyzed by detection of nucleocapsid protein by immunofluorescence and Western blot. Equal loading in cellular lysates uninfected and HTNV-infected cells was controlled by detection of tubulin. Release of viral particles was analyzed by detection of nucleocapsid protein in cell-free supernatants of infected cells by Western blot and detection of viral nucleocapsid protein in Vero E6 and MDCKII cells re-infected with supernatants of infected MDCKII cells.