Figure 3
From: Polar release of pathogenic Old World hantaviruses from renal tubular epithelial cells
Expression of hantavirus receptors CD55 and integrin α V β 3 in MDCKII cells. A) Polarized MDCKII cells were analyzed for the expression of ZO-1 (green) and β-catenin(red) and analyzed by confocal microscopy. Nuclei (blue) were stained with Hoechst 33342. B) Electron microscopy revealed polarization of MDCKII cells cultured on transwell filters. Upper panel: Transmission electron micrographs of polarized MDCKII cells exhibited cells growing a confluent monolayer with junctional complexes (tight junction and adherens junction, respectively indicated by white arrow and a desmosome indicated by arrowhead) at cell-to-cell contact sites. Lower panel: Scanning electron micrographs of MDCKII cells exhibited a confluent cell layer with the apical formation of a typical dense meshwork of microvilli. Scale bars: upper panel: 500 nm, lower panel 2 um. C) Surface expression of CD55 and integrin αVβ3. MDCKII cells were stained with anti-CD55-APC antibody and anti-integrin αVβ3-PE antibody and analyzed by flow cytometry.