Evaluation of nanoparticle tracking analysis for total virus particle determination

Table 1 Analysis of 120 nm latex particles

Dilution	Measurement No.	Particles/frame	Completed tracks	Mean particle size (nm)	Measured conc.(LP/ml)	Average measured conc. (LP/ml)	Concentration (LP/ml)	Measured factor (MF)	Theoretical factor (TF)	TF/MF
300	1	91*	2896	115	1,52E+09
300	2	79*	2407	119	1,27E+09	1,4E+09	4,2E+11
500	1	120*	4179	100	1,93E+09			0,7	1,7	2,3
500	2	115*	3999	103	1,86E+09	1,9E+09	9,5E+11
900	1	87*	2621	125	1,40E+09			1,4	1,8	1,3
900	2	80*	2253	133	1,29E+09	1,3E+09	1,2E+12
1000	1	64*	2004	125	1,03E+09
	2	35	755	143	5,69E+08
	3	23	575	131	3,78E+08	4,7E+08	4,7E+11
1500	1	61*	2005	106	9,92E+08			0,6	1,5	2,6
1500	2	52	1688	110	8,35E+08	8,4E+08	1,3E+12**
2000	1	29	748	130	4,71E+08			1,7	1,3	0,8
2000	2	33	1004	109	5,38E+08	5,0E+08	1,0E+12**
3000	1	13*	343	149	2,16E+08			1,3	1,5	1,2
	2	24	640	146	3,90E+08
	3	24	681	127	3,94E+08	3,9E+08	1,2E+12**
							1,15E+12	Final concentration (LP/ml)

* Measurements where the “particle/frame” was higher than 60 or lower than 20 (consequently these measurements were eliminated from final calculation).
**Concentration values with TF/MF factor between 0.8 and 1.2 (these values were used to calculate the original concentration of latex particles).

ISSN: 1743-422X