Figure 1

Northern blot analysis of the transcripts of CLCuBuV. Northern blot of total RNA extracted from a healthy Nicotiana benthamiana plant (H) and a plant infected with CLCuBuV/CLCuMuB (I). Detection of complementary- and virion-sense specific RNAs in infected plants are shown in panels A and B respectively. Immobilized RNA was hybridized to complementary- (A) and virion-sense probes (B). Detection of CLCuMuB specific RNA is shown in panel C. Approximately equal amounts (10μg) of RNA were loaded in each well. Estimated sizes are given in kilobases (kb) based on the positions of a co-electrophoresed RNA marker (M). Two additional hybridization signals (indicated by the asterisks) and the smear at the bottom of the virion-sense probed blot (indicated by the bracket on the right) is discussed in the text.