Figure 2From: Frequency of subtype B and F1 dual infection in HIV-1 positive, Brazilian men who have sex with menRepresentative agarose gel of the nested polymerase chain reaction (PCR) produts. Electrophoresis and subsequent ethidium bromide staining of amplicons from the nested PCR amplification with pol-IN subclade F1-specific primers of the DNA samples known to harbor HIV-1 subclade F1 (A), and subtype B (B). Amplicons of nested PCR (1247 bp) performed with L-pol subclade F1-specific primers using samples that were observed to be infected with HIV-1 subclade F1 (solid line), BF1 recombinants with breakpoints located between the PCR primers (dotted line), and subtype B (dashed line). (C). Amplicons of nested PCR (727 bp) performed with M-pol primers (specific for F1 and BF1 variants) using samples observed to be infected with HIV-1 subclade F1 (solid line), BF1 recombinants with breakpoints located between the PCR primers (dotted line), and subtype B (dashed line) (D). M, molecular weight DNA marker (100 bp DNA ladder, Invitrogen).Back to article page