Table 1 Characterization of the fluorescent molecules used to tag HCMV tegument proteins pp65, pp71, pp150, and pp28
From: Human cytomegalovirus tegument proteins (pp65, pp71, pp150, pp28)
Fluorescent Molecule | Tegument Protein(s) Labeled | Excitation Peak (nm) | Emission Peak (nm) | Brightness | Photostability | pKa |
|---|---|---|---|---|---|---|
Cyan | pp65 | 433/445 | 475/503 | 13 | 64 | 4.7 |
RFP | pp71 | 584 | 607 | 12.5 | 8.7 | 4.5 |
GFP | pp150 and pp28 | 488 | 507 | 34 | 174 | 6.0 |