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Figure 5 | Virology Journal

Figure 5

From: Real-time RT-PCR high-resolution melting curve analysis and multiplex RT-PCR to detect and differentiate grapevine leafroll-associated virus 3 variant groups I, II, III and VI

Figure 5

Agarose gel electrophoresis of multiplex RT-PCR amplicons. Visualization of multiplex RT-PCR amplicons separated on a 2% TAE agarose gel with ethidium bromide staining. Figure5A represents grapevine samples singly infected with one GLRaV-3 variant group and Figure5B represents field samples with multiple infections. Lane 1: Fermentas GeneRuler 100 bp DNA ladder, Lane 2: Group II variant (563 bp) and V. vinifera actin (82 bp), Lane 3: Group I variant (429 bp) and V. vinifera actin, Lane 4: Group III variant (314 bp) and V. vinifera actin, Lane 5: Group VI variant (179 bp) and V. vinifera actin, Lane 6: Group I, II, III and VI variants and V. vinifera actin, Lane 7: Negative control, Lane 8: Fermentas GeneRuler 100 bp DNA ladder, Lane 9: Group II, VI and V. vinifera actin, Lane 10: Group II, III, VI and V. vinifera actin, Lane 11: Group II, III, VI and V. vinifera actin, Lane 12: Group II, III, VI and V. vinifera actin, Lane 13: Group II, III, VI and V. vinifera actin , Lane 14: Group I, II, III, VI variants and V. vinifera actin.

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Virology Journal

ISSN: 1743-422X