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Figure 3 | Virology Journal

Figure 3

From: One-step detection of Bean pod mottle virusin soybean seeds by the reverse-transcription loop-mediated isothermal amplification

Figure 3

Optimization of reaction temperature of the RT-LAMP for the detection of BPMV in soybean seeds. RT-LAMP was carried out at 60, 62.5, 65, 67.5 and 70°C to detect BPMV in soybean seeds. 3 μL of amplified products were observed on a 1% agarose gel electrophoresis. M: 2 kb DNA marker; 1 and 2: Soybean seeds harboring BPMV (sample 1 and 2): 3: Healthy soybean seeds; 4: Negative control without DNA template.

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Virology Journal

ISSN: 1743-422X