Figure 5

Gel electrophoresis of the PCR product of MUC7 tandem repeat regions showing variations in the number of tandem repeats. A 1% agarose gel was used for MUC7 DNA samples. 20ul of reaction mixture was loaded for each sample into each lane. MWM marks those lanes loaded with a DNA molecular weight marker. Ethidium bromide enabled DNA to be viewed under ultraviolet light. Lanes 1–10 are HIV negative DNA samples and 11–20 HIV positive DNA samples (a representation of samples is shown, lanes are numbered as such for clarity purposes). A single band (590 bp) represents the tandem repeat fragment with six repeats indicating a donor who is homozygous for MUC7. The double band, marked with an arrow, is of the fragment with six repeats and the smaller sized band (521 bp) is the fragment with five repeats. This indicates a heterozygous genotype for the donor for MUC7.