Figure 1

The gel filtration profile of crude saliva from an HIV negative donor. Crude saliva (20 ml in 6 M GuHCl, 10 mM EDTA, 1 mM PMSF pH 6.5) from an HIV negative donor was chromatographed on a Sepharose CL-4B gel filtration column (bed volume 200 ml). The column was eluted with 0.2 M NaCl: 0.02% NaN₃ at a flow rate of 60 ml/h at room temperature. An aliquot of each fraction was assayed by the PAS and Lowry methods. V₀ indicates the void volume and V_i indicates the included volume of the column. Fractions 18–34 were pooled to recover MUC5B and fractions 50–66 were pooled to recover MUC7. Fractions were dialysed against three changes of distilled water overnight at 4°C, and freeze-dried.