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Figure 1 | Virology Journal

Figure 1

From: In vitroinfection with classical swine fever virus inhibits the transcription of immune response genes

Figure 1

Detection of CSFV on the infected PK-15 cells. Figure 1–1 CSFV genomic RNAs present in PK-15 cells at 1, 3, 12, 24, 36, and 48 h post-inoculation with different MOI of CSFV strain Shimen. Viral genome copy number was assessed by real-time RT-PCR. Figure 1–2 RNA from PK-15 cells inoculated with the Shimen strain of CSFV. (a) GAPDH gene detection from PK-15 cells inoculated with Shimen strain of CSFV; (b) Total RNA detection from PK-15 cells inoculated with Shimen strain of CSFV. Lanes 1, 4, 7, 10, 13, and 16 show the mock PK-15 cells cultures at 1, 3, 12, 24, 36, and 48 h post-inoculation with CSFV. Lanes 2, 5, 8, 11, 14, and 17 show PK-15 cells culture inoculated with 0.1 MOI of CSFV at 1, 3, 12, 24, 36, and 48 h. Lanes 3, 6, 9, 12, 15, and 18 show PK-15 cells inoculated with 1.0 MOI of CSFV at 1, 3, 12, 24, 36, and 48 h.

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Virology Journal

ISSN: 1743-422X