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Figure 2 | Virology Journal

Figure 2

From: Experimental chronic hepatitis B infection of neonatal tree shrews (Tupaia belangeri chinensis): A model to study molecular causes for susceptibility and disease progression to chronic hepatitis in humans

Figure 2

HBV cccDNA in liver detected by nPCR. M: Molecular size standard, 100 bp ladder. 1: Negative control, sterile water. 2-5: Liver biopsies of tree shrews. Lanes 2, 3 and 5 showed positive bands at 356 bp. These three positive samples were collected from animal no. 90-1 at 2 years and 3 moths, no. 97-1 at 48 weeks and no. 98-2 at 42 weeks after inoculation, respectively. Sequence analysis showed they correspond to the sequence of HBV. 6: Positive control of HBV cccDNA, recombinant plasmid pUC18-HBV.

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