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Figure 2 | Virology Journal

Figure 2

From: Identification of nonessential regions of the nsp2 protein of an attenuated vaccine strain (HuN4-F112) of highly pathogenic porcine reproductive and respiratory syndrome virus for replication in marc-145 cell

Figure 2

Construction of PRRSV strain HuN4-F112 nsp2 mutants. Mutagenesis PCR was used to delete and insert fragment in the nsp2 region. The deleted amino acids are shown as thin dashed lines, thick dashed lines indicate the sequence naturally deleted as compared with the North American genotype representative virus VR-2332. The putative enzyme domain (PL2) and TM region are indicated in the figure. The viability for each mutant constructed is shown on the right: +, viable; -, nonviable.

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Virology Journal

ISSN: 1743-422X