Table 1 Primer sets designed to detect hMPV N gene by RT-LAMP
Primer | Sequence | Genome position | Length |
|---|---|---|---|
F3 | ACAGGAGTCTATTCATTGAGT | 659–679 | 20 |
B3 | ACCAAATCATAAACCTCTGTG | 855–875 | 18 |
FIP(F1c + F2) | CGGCTCCATAAGCTTGCATAAATAT-GGGAAAGCTTTAGGCTCA | (739–763) + (682–699) | 22 + 20 |
BIP(B1c + B2) | ACAATGCTAAGGTGGGGTGTC-CTTCAATTCAGCTTGCACAG | (770–789) + (830–849) | 20 + 20 |
LF | CAAACAAACTTTCTGCTTTGCTTCC | 709–733 | 20 |
LB | CATCTAACAACATAATGCTAGGGCA | 800–824 | 25 |