Genome and proteome analysis of 7-7-1, a flagellotropic phage infecting Agrobacterium sp H13-3

Table 1 Overview of the structural proteins identified by ESI-MS/MS

Protein number	Protein name	Protein MW (Da)	Max. No. of unique spectra	Maxi. sequence coverage (%)	Slice in which most abundant	Remarks
gp2	Conserved hypothetical protein	81,838	28	53.9%	5
gp3	Putative DNA circulation protein	43,673	6	22.6%	9
gp6	Conserved hypothetical protein	19,283	6	43.3%	20
gp7	Baseplate protein; phage P2 GpJ homolog	43,379	11	43.9%	9
gp8	Hypothetical protein	31,431	5	27.3%	13
gp9	Hypothetical protein	31,400	4	23.2%	13 -14
gp102	Putative tail fibre	61,504	6	17.7%	4
gp103	Hypothetical protein	14,237	4	44.7%	22
gp106	Hypothetical protein	14,221	7	51.7%	23
gp107	Hypothetical protein	52,046	18	36.9%	1
gp108	Hypothetical protein	37,504	3	13.5%	11
gp111	Hypothetical protein	4,295	2	45.2%	25
gp113	Portal protein	45,459	10	34.2%	9
gp114	prohead protease	28,590	2	10.5%	18
gp115	Major capsid protein	52,513	19	46.5%	11 -13 - 14	only 'C-terminal' sequence coverage
gp116	Hypothetical protein	14,261	3	37.9%	20 - 21 - 22
gp117A	Hypothetical protein	3,205	4	85.7%	25
gp118	Hypothetical protein	24,820	9	44.3%	14
gp119	Hypothetical protein	22,041	9	84.7%	17
gp121	Hypothetical protein	15,986	3	35.0%	19
gp122	Hypothetical protein	23,047	6	20.5%	15
gp124	Hypothetical protein	20,253	1	8.4%	18	protein identification probability of 87.70%
gp126	Tail sheath protein	54,066	21	50.5%	7
gp127	Hypothetical protein	14,475	5	50.7%	18

For every detected protein the protein name, the predicted molecular weight (Da), the maximum number of unique spectra and sequence coverage (%) is listed. Moreover, the gel slice in which those maximums were observed is given, as well as some additional remarks.

ISSN: 1743-422X