Figure 3

Neutrophil influx and CXCL2 expression were lower in neonatal lungs. Adult and neonatal C57BL/6 mice were inoculated with SeV 500 pfu/g body weight. a) On the indicated days, BAL was performed and total BAL fluid cells were counted. b) To enumerate the neutrophils, BAL fluid cells were subjected to Wright Giemsa staining and differential cell counting. c) Percentage of neturophils of total BAL cells. d) Whole lung RNA was analyzed by real-time PCR for CXCL2. *p < 0.05. Whole lung protein homogenates were analyzed for e) CXCL2, f) IFN-γ, g) TNF-α, and h) IL-6 protein by ELISA. *p < 0.05. White bars denote adults, and black bars denote neonates. N = 4 at each time point. All values represent the mean ± SD