Table 1 Real-time PCR quantification of different target sequences in virus isolates obtained by the marker rescue procedure
From: Selection of recombinant MVA by rescue of the essential D4R gene
Template | Total MVA(1) | YF prME(1) | lacZ(1) | AmpR(1) |
|---|---|---|---|---|
rMVA-YF/D4 #1 | 8.54 | 8.44 | 3.33 | 3.83 |
rMVA-YF/D4 #2 | 8.38 | 8.25 | 3.17 | 4.02 |
rMVA-YF/D4 #3 | 8.54 | 8.30 | 3.47 | 4.02 |
rMVA-YF/D4 #4 | 8.58 | 8.24 | 3.37 | 4.07 |
rMVA-YF/D4 #5 | 8.69 | 8.42 | 3.37 | 3.89 |
rMVA-YF/D4 #6 | 8.33 | 8.14 | 3.45 | 4.08 |
rMVA-YF/D4 #7 | 8.64 | 8.49 | 3.66 | 4.11 |
rMVA-YF/D4 #8 | 8.54 | 8.50 | 3.61 | 4.02 |
rMVA-YF/D4 #9 | 8.74 | 8.55 | 3.48 | 3.97 |
rMVA-YF/D4 #10 | 8.39 | 8.27 | 3.54 | 4.07 |
rMVA-YF/D4 #11 | 8.75 | 8.49 | 3.42 | 3.96 |
rMVA-YF/D4 #12 | 8.46 | 8.44 | 3.56 | 4.06 |
Uninfected cDF-1 | 0.00 | 0.00 | 3.58 | 4.01 |
Wild-type MVA | 8.41 | 2.62 | 3.27 | 3.93 |
dMVA-ZG | 7.46 | 3.57 | 7.34 | 3.93 |