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Figure 3 | Virology Journal

Figure 3

From: Rotavirus nonstructural protein 1 antagonizes innate immune response by interacting with retinoic acid inducible gene I

Figure 3

Analysis of interaction between NSP1 and RIG-I. (A) Immunoprecipitation analysis for the association between OSU NSP1 and RIG-I. 293FT cells were transfected with plasmids encoding full-length Myc-RIG-I, GFP, and GFP-OSU NSP1. At 48 h after transfection, cell lysates were immunoprecipitated (IP) with antibodies against Myc, and detected by SDS-PAGE. Immunoprecipitates and aliquots of cell lysates were then assayed by Western blot (WB) analysis. (B) SA11 NSP1 interaction with RIG-I. 293FT cells were transfected with plasmids encoding Myc-RIG-I, GFP-SA11 NSP1, and GFP separately. Immunoprecipitation and Western blot analysis were performed as described for panel A. (C) Interaction between NSP1ΔIRF3BD of rotavirus SA11 and RIG-I. Transfection, immunoprecipitation and Western blot analysis were performed as described above.

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Virology Journal

ISSN: 1743-422X