Figure 3
From: RNA binding properties of the US11 protein from four primate simplexviruses
Determination of Kd of binding to dsRNA of US11 protein from HSV-1. The Kd was calculated by non-linear regression as described in Materials and methods and it is reported on the figure together with the estimated standard error. A) The Kd was determined with a fixed concentration of dsRNA (1 nM) and a range of concentration of proteins from 1 to 100 nM. B) The Kd was determined with a fixed concentration of protein (2 nM) and a range of concentrations of dsRNA from 0.97 to 125 nM.