Figure 2From: Inhibition of highly pathogenic PRRSV replication in MARC-145 cells by artificial microRNAsDesigned amiRNAs and target sites selection using luciferase assay. Five amiRNAs were designed via online tools of Invitrogen Company (A). Transfection efficiency assay via GFP monitoring after co-transfected with plasmids expressing amiRNAs that fused to GFP coding gene and psiCHECK2-GP5 or -M. Images were taken with an inverted fluorescence microscope in bright (left) or fluorescence (right) light of the same field. Magnification, 40× (upper) or 100 × (bottom) (B). Inhibition of Renilla luciferase expression by amiRNAs that targeting PRRSV GP5 or M protein coding sequences were measured by duel luciferase assay at 48 hours post-cotransfection. The Renilla luciferase data has been normalized to firefly luciferase data. Normalized Renilla luciferase expression in the psiCHECK2 only transfection was set at 100%. Data are mean ± SD, n = 3~4, *p < 0.05 vs. psiCHECK alone transfected cells group (C, D).Back to article page