Figure 1

Effect of HIF-1α on HIV-1 LTR. A and B. 786-O cells were transfected with 0.1 μg of the LTR-Luc full length or deletion mutants along with an increasing concentration of HIF-1α (A) or 0.5 μg of HIF-1α (B). The amount of DNA in each transfection mixture was normalized with pcDNA₆HisA. Luciferase activity was determined 48 hours after transfection. Results are displayed as histograms. C. Approximately 100, 000 cpm of synthetic [γ³²P]-labeled double-stranded DNA oligonucleotide probe corresponding to the HIV-1 LTR GC-rich site was incubated with 10 μg of nuclear extracts prepared from microglial cells transfected with c-myc-HIF-1α. Labeled probe was also incubated with nuclear extracts prepared from pcDNA-transfected microglial cells in the presence of a specific DNA competitor (cold probe, lane 3), non-specific competitor (an unrelated dsDNA) (lane 4), anti-c-myc antibody (lane 6) and normal mouse serum (NMS) (lane 5).