Reactions of Nicotiana species to inoculation with monopartite and bipartite begomoviruses

Table 1 Summary of the results of the infectivity studies

Species (ploidy level, chromosome number)	Parents	Origin	Infectivity of ToLCNDV (plants symptomatic/plants inoculated)	Plants PCR positive for ToLCNDV with primers ToLCNDVV2F/ToLCNDVV2R	Infectivity of CLCuMV (plants symptomatic/plants inoculated)	Plants PCR positive for CLCuMV with primers PK3AV2F/PK3AV2R	Plants PCR positive for CLCuMV with primers BegomoF/BegomoR
N. sylvestris (2x = 24)	-	South America	4/4	4	0/4	0	0 (0.7)*
N. obtusifolia (2x = 24)	-	Southwestern USA, Mexico	0/4	0	0/4	0	0 (1.4)*
N. benthamiana (4x = 38)	N. sylvestris (likely maternal parent)	Australia	4/4	4	4/4	4	4 (2.8)*
N. suaveolens (4x = 32)	N. sylvestris (likely maternal parent)	Australia	0/4	0	0/4	0	0
N. tabacum (4x = 48)	N. tomentosiformis, N. sylvestris	Eastern North America	4/4	4	0/4	4	4 (2.8)*
N. nudicaulis (4x = 48)	N. obtusifolia, N. sylvestris	North America	0/4	4	0/4	2	2 (2.8)*
N. repanda (4x = 48)	N. obtusifolia, N. sylvestris	Southern Texas (USA)	0/4	4	0/4	0	0 (1.4)*

*The sizes of bands produced in PCR reactions with primers BegomoF/BegomoR are shown in kb. The expected size product is 2.8kb for a full CLCuMV genome.

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