Genetic evolution of low pathogenecity H9N2 Avian influenza viruses in Tunisia: acquisition of new mutations

Table 1 Primer sequences as used in the RT-PCR in our study

Name	Sequence (5'- 3')^a	Position	Expected products size (pb)	Reference
AMF	CTTCTAACCGAGGTCGAAAC	7- 26	244	15
AMR	AGGGCATTTTGGACAAAKCGTCTA	259-238
MF	CTCATGGAATGGCTAAAGACA	149-169	700	16
MR	CGATCAADAATCCACAATATC	847-827
H9-1	CTYCACACAGARCACAATGG	184-203	488	15
H9-2	GTCACACTTGTTGTTGTRTC	672-652
H9F	GAATCCAGATCTTTCCAGAC	426-445	384	17
H9R	CCATACCATGGGGCAATTAG	808-789
NPF	CAGRTACTGGGCHATAAGRAC	1200-1220	326	18
NPR	GCA TTGTCTCCGAAGAAATAAG	1529-1510
HAF1	GAATTGATTATTATTGGTCAGTA	710-732	550	19
HAR1	TCATCAATCT-TATTGTTGATCAT	1272-1249
NAF	CTTGTTGGCGACACACCAAGRAA	961-983	410	19
NAR	GAGCCTGTTCCAT-AGGTACCTGA	1370-1348
PB2F	TATTCAT-CRTCAATGATGTGGGA	1591-1613	540	19
PB2R	GATGCTYAATGCTGGTCCATATC	2130-2108
NSF	AGCAAAAGCAGGGTGACAAA	1-20	890	20
NSR	AGTAGAAACAAGGGTGTTTT	890-871

^a Codes for mixed bases positions: D = G/A/T, H = A/C/T, K = G/T, R = A/G, Y = C/T.

ISSN: 1743-422X