From: The development of a rapid SYBR Green I-based quantitative PCR for detection of Duck circovirus
Concentration of standard plasmid (DNA copies/μL) | Intra-assay variability | Inter-assay variability | ||||
---|---|---|---|---|---|---|
C T | CV (%) | C T | CV (%) | |||
Mean | SD | Mean | SD | |||
1.31 × 107 | 13.67 | 0.26 | 1.89 | 13.59 | 0.17 | 1.26 |
1.31 × 106 | 17.66 | 0.19 | 1.07 | 17.62 | 0.13 | 0.74 |
1.31 × 105 | 21.19 | 0.03 | 0.16 | 21.20 | 0.04 | 0.19 |
1.31 × 104 | 25.00 | 0.13 | 0.50 | 25.06 | 0.08 | 0.32 |
1.31 × 103 | 27.22 | 0.17 | 0.62 | 27.23 | 0.20 | 0.72 |
1.31 × 102 | 30.61 | 0.18 | 0.60 | 30.41 | 0.34 | 1.10 |