Establishing a TaqMan-Based Real-Time PCR Assay for the rapid detection and quantification of the newly emerged duck tembusu virus

Table 4 Quantification of DTMUV in tissue samples by real-time PCR^a and virus isolation^b

Samples	Duck 1		Duck 2		Duck 3		Duck 4
	Real-time PCR	Virus isolation^b	Real-time PCR	Virus isolation^b	Real-time PCR	Virus isolation^b	Real-time PCR	Virus isolation^b
Blood	6.56 ± 0.06	2.50	4.46 ± 0.36	0.98	-	-	6.80 ± 0.03	1.25
Liver	4.88 ± 0.03	-	4.53 ± 0.08	-	5.54 ± 0.05	0.97	6.66 ± 0.02	2.17
Lungs	5.49 ± 0.04	2.75	5.02 ± 0.01	1.38	6.11 ± 0.02	3.25	6.48 ± 0.01	3.50
Kidneys	5.01 ± 0.02	1.75	4.02 ± 0.06	-	5.49 ± 0.15	3.50	5.42 ± 0.05	2.75
Brain	-		5.04 ± 0.06	1.75	5.43 ± 0.01	2.50	6.50 ± 0.08	2.25
Pancreas	6.75 ± 0.04	2.25	6.91 ± 0.01	2.50	7.52 ± 0.02	1.67	7.56 ± 0.02	3.25

(-): Negative results with the real-time PCR or virus isolation assay.
Ducks 1 and 2 were inoculated intranasally with 10⁵ ELD₅₀ FXV and euthanized 4 and 7 days post-inoculation, respectively.
Ducks 3 and 4 were introduced into the isolator, placed with the intranasally inoculated ducks, and euthanized 6 days post-contact.
^a Unit: log 10 copies/mL for blood and log 10 copies/g for other tissue samples.
^b Unit: log 10 ELD₅₀/mL for blood and log 10 ELD₅₀/g for other tissue samples.

ISSN: 1743-422X