Establishing a TaqMan-Based Real-Time PCR Assay for the rapid detection and quantification of the newly emerged duck tembusu virus

Table 2 Virus detection from oropharyngeal and cloacal swabs of the experimentally infected ducks by real-time PCR and virus isolation

Samples	Days PI ^c	Oropharyngeal swabs			Cloacal swabs
		Real-time PCR		Virus isolation	Real-time PCR		Virus isolation
		C t^a	Result^b		C t^a	Result^b
1	2	> 35	-	-	> 35	-	-
2	2	33.67 ± 2.60	+	-	33.58 ± 0.61	+	+
3	3	33.59 ± 0.05	+	-	32.79 ± 0.10	+	-
4	3	28.21 ± 0.09	+	+	28.37 ± 0.19	+	-
5	5	32.06 ± 0.29	+	+	27.34 ± 0.12	+	-
6	5	31.10 ± 0.33	+	-	25.46 ± 0.22	+	+

(+): positive; (-): negative
^a C t values obtained for the experiment are expressed as mean ± standard deviation (n = 3) if greater than zero.
^b C t < 35 was considered positive (+).
^c PI: post-inoculation.

ISSN: 1743-422X