Figure 2

Expression of encapsidated CAT RNA in mammalian cell lines. CAT expression levels are shown in BHK-21 (I), HeLa (II) and HEK 293 (III) cell lysates 24 hours after unheated or heat treated VLP uptake. Gag VLPs containing 10 ng of CAT RNA were used for the cell uptake assays in each cell line. Heat treated VLPs were incubated for 20 min at 62°C. Error bars display the variance between 3 separate experiments. P values are displayed above each set of uptake assays.