Figure 2
Antibody responses after vaccination with individual poxvirus DNA vaccines administered using muscle electroporation. A. Sera collected from three rabbits vaccinated with pWRG/A33R, or pWRG/B5R, or pWRG/A27L, or pWRG/TPA-L1R(opt) were tested for antibodies that bind A33, B5, A27, and L1 by immunogen-specific ELISA. Symbols represent mean endpoint ELISA titers ± SE. The limit of detection was a titer of 2 log10 (dashed line). B. Sera from rabbits vaccinated with the MV-specific targets, L1 and A27, were tested for VACV neutralizing antibodies by PRNT. Titers for each time-point are shown for individual rabbits. The limit of detection was a titer of 20 (dashed line). C. Day 70 sera from rabbits vaccinated with the EV-specific targets, A33 and B5, were tested for their capacity to prevent the spread of EV in an EV spread inhibition assay. Sera from rabbits vaccinated with pWRG/A27L were included as negative controls. Other controls included wells that were overlain with methylcellulose after the adsorption step (no EV spread) and well that received media without dilute sera (no sera control). Symbols represent sera diluted 1:14-1:56. Numbers of satellite plaques per well are shown as scatter graphs. The mean ± SD for each serum sample is shown. Rabbit ID # and the plasmid vaccine used to generate the sera are shown on the x-axis. D. Sera from rabbits were diluted and samples were incubated with fresh EV particles in the presence or absence of complement as indicated. EV neutraliation titers were determined as described in the material and methods. The limit of detection was a titer of 40 (dashed line).