From: IPNV with high and low virulence: host immune responses and viral mutations during infection
Genes | Assay | Primer | Sequence (5'-3') | PCR efficiency | GenBank accession # |
---|---|---|---|---|---|
EF1aB | TaqMan | Forward Reverse Probe | TGCCCCTCCAGGATGTCTAC CACGGCCCACAGGTACTG AAATCGGCGGTATTGG | 2.0 | BG933897 |
IFN-c | TaqMan | Forward Reverse Probe | TGGGCAGTGTGGATACAAGTG CTGCAATGTTCCCAAAGTACGTATT CTGTCCTGATGAGATAAT | 2.0 | EU735545 EU735547-50 |
IFN-a1 | TaqMan | Forward Reverse Probe | CCTTTCCCTGCTGGACCA TGTCTGTAAAGGGATGTTGGGAAAA CTTTGTGATATCTCCTCCCATC | 1.94 | AY2169594 AY2169595 |
IFN-γ | TaqMan | Forward Reverse Probe | AAGGGCTGTGATGTGTTTCTG TGTACTGAGCGGCATTACTCC TTGATGGGCTGGATGACTTTAGGA | 2.0 | AY795563 |
Mx1/2 e/e | TaqMan | Forward Reverse Probe | GATGCTGCACCTCAAGTCCTATTA CGGATCACCATGGGAATCTGA CAGGATATCCAGTCAACGTT | 2.0 | U66475 U66476 |
MyD88 | TaqMan | Forward Reverse Probe | GACAAAGTTTGCCCTCAGTCTCT CCGTCAGGAACCTCAGGATACT CTGGTGCCCGGAGCAA | 1.84 | EF672332 |
TLR8 | TaqMan | Forward Reverse Probe | ACCAAAACCACTAATGACATCATCTTCA TGGTGATGCCATCAGGTATGTTT CTCAGTCGACGCTCCTC | 1.7 | FJ467615 |
TLR9 | TaqMan | Forward Reverse Probe | TCTATGGCTGGGATGTCTGGTA CAGTTGTGAGTAGCCCTTGTGT CAGCACCTGGAAGCAG | 1.81 | EF672331 |
TLR22 | TaqMan | Forward Reverse Probe | ATTTATCCCGGAATCCATGTATCACG CCACAGTAGGCGATGTCTAACA CCTCAAGATAAGGAAGAACAT | 1.98 | AM233509 |
VP2 (IPNV) | TaqMan | Forward Reverse Probe | GCCAAGATGACCCAGTCCAT TGACAGCTTGACCCTGGTGAT CCGACCGAGAACAT | 2.0 | AJ877117 |
Beta 2 microglobulin | SybrGreen | Forward Reverse | TCGTTGTACTTGTGCTCATTTACAGC CAGGGTATTCTTATCTCCAAAGTTGC | 1,6 | BX076608 |
Galectin-3 binding protein | SybrGreen | Forward Reverse | CCAGACCAACAGTGTTCACTTCAGC ACGTGAAAGACATACCTGCCCTCAC | 1,84 | BX079375 |
Galectin-9 | SybrGreen | Forward Reverse | GTCCTGTCTATTGCCTTCTCCAACC GGTTTCGTTGACCACTGTGTGGA | 1,76 | CU071943 |
MHC-IHC | SybrGreen | Forward Reverse | CTGCATTGAGTGGCTGAAGA GGTGATCTTGTCCGTCTTTC | 1,72 | CU070775 |
Pre-B cell ef | SybrGreen | Forward Reverse | GACTTCAATTTCCTGCTGGCTA CTGCTTGTAATGTGTGACCT | 1.77 | CA376673 |
RIG-I | SybrGreen | Forward Reverse | GACGGTCAGCAGGGTGTACT CCCGTGTCCTAACGAACAGT | 1.97 | DY714827 |
MDA5 | SybrGreen | Forward Reverse | CAGAGGTGGGGTTCAATGAT AGCTCGCTCCACTTGTTGAT | 1.92 | FN396357 |
PKR | SybrGreen | Forward Reverse | TTCCTGCATGGACTTGACTG GTGAGGAACCGGTGTTCTGT | 1.87 | BT046111 |
SRK2 | SybrGreen | Forward Reverse | TAGACATGGCACCATGGACCCTC GGGTTCTTCAGTGCAGACAGCCA | 1.81 | CA349577 |