A computational approach to identify point mutations associated with occult hepatitis B: significant mutations affect coding regions but not regulative elements of HBV

Table 2 Nucleotide positions significantly enriched in OBI dataset by univariable analysis

Nucleotide positions*	Adjusted p-value	Significant nt changes in OBI vs. non-OBI	Amino acid change in affected *open reading frames*				Affected HBV protein**
			polymerase	surface		core
				pre-S2	S
78	0.0144	G → A	no change	Gly → Glu			L, M
233	0.0367	A → G	His → Arg (RT domain)		Thr → Ala		P, L, M, S
418	0.0367	G → T	Ala → Ser (RT domain)		no change		P
2240 ***	0.0097	A → G				Thr → Val (T helper epitope) ***	C
2241 ***	0.0144	C → T				Thr → Val (T helper epitope) ***	C
2435	0.0367	C → A	no change			Gln → Lys (Arg-rich C-terminus)	C
2485	0.0367	A → G	Asn → Ser (TP domain)				P

* positions in the well-annotated reference HBV sequence Acc.No. AM282986
** L, M, S: Large, Middle and Small S, respectively; P: Polymerase; C: Core
*** nucleotides 2240 and 2241 co-variated in all observed cases, substituting ACT with GTT or ACA with GTA codons.

ISSN: 1743-422X