Figure 6

Immunostaining of infected Huh7.5 cells with anti p412 caprine antibody. Huh7.5 cell lines were infected with positive HCV sera as described in Materials and Methods. Immunostaining was carried out after 5 days from infection. Anti p412 was used as a primary antibody and anti-caprine peroxidase as a secondary antibody. DAB was used as substrate. Brown foci were visualized in infected cells (B) but not in non infected cells (A).