Figure 3From: Japanese Encephalitis Virus wild strain infection suppresses dendritic cells maturation and function, and causes the expansion of regulatory T cellsCytokine profiles of P3-infected DCs (IFN-α, TNF-α, CCL2 and IL-10). 1 × 105 freshly purified bmDCs were left mock-treated or treated with 1 MOI of P3 or UV-P3 for 3 days. The spDCs from mice, which were challenged or immunized for 5 days, were obtained and cultured for 3 days. The cell supernatants harvested at 3 days of post infection were analyzed with ELISA to measure the concentrations of cytokines (IFN-α, TNF-α, CCL2 and IL-10). Cytokine concentrations were expressed as the means ± SD of triplicates. *, P < 0.05; **, P < 0.01.Back to article page