A novel synthetic quantification standard including virus and internal report targets: application for the detection and quantification of emerging begomoviruses on tomato

Table 2 Reaction conditions and assay performance of the five duplex real-time PCRs

Target	Virus		Internal report (ADN 25S)		T°m (°C)	Linear dynamic range (copies/uL)		PCR efficiency (%)
	Primers	Probe	Primers	Probe		Virus	Internal report	Virus	Internal report
TYLCV-IL	650	150	500	100	63	2 × 10⁹ to 2 × 10³	2 × 10⁷to 2 × 10³	96	108
TYLCV-Mld	250	200	750	150	64	2 × 10⁹to2 × 10³	2 × 10⁹to2 × 10³	103	107
PYMV-A	750	150	200	50	63	2 × 10⁸to2 × 10³	2 × 10⁷to2 × 10³	93	100
PYMV-B	900	150	200	50	63	2 × 10⁹to2 × 10³	2 × 10⁹to2 × 10³	97	100
ToLCKMV-like	750	150	300	50	62	2 × 10⁸to2 × 10³	2 × 10⁷to2 × 10³	95	104

ISSN: 1743-422X