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Figure 1 | Virology Journal

Figure 1

From: Development of an in situ assay for simultaneous detection of the genomic and replicative form of PCV2 using padlock probes and rolling circle amplification

Figure 1

Schematics over the padlock probe and rolling circle amplification procedure. Strand-specific padlock probes are hybridised and ligated a) directly on single stranded DNA (for the genomic form), and b) after restriction digestion and exonucleolysis on double stranded DNA (for the replicative form). The target DNA is then cut site-specifically using the combined action of MutY and EndoIV enzymes to create a free 3'-end as a starting point for RCA. After RCA the rolling circle products are visualised by hybridisation of fluorescently labelled detection oligonucleotides, green for the products from the probe specific for the genomic strand and red for the probes specific for the replicative form.

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Virology Journal

ISSN: 1743-422X