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Figure 2 | Virology Journal

Figure 2

From: Functional processing and secretion of Chikungunya virus E1 and E2 glycoproteins in insect cells

Figure 2

N-linked glycosylation of CHIKV E1 and E2 synthesized with baculovirus vectors. A) Infected Sf21 cells were treated with tunicamycin and cell fractions were analyzed by western blotting using α-E1 and α-E2 polyclonal antisera. B) Glycosylation of recombinant CHIKV E1 and E2 was confirmed by PAS-staining. C) Detection of baculovirus glycoprotein GP64 verified tunicamycin activity.

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Virology Journal

ISSN: 1743-422X